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Effect of GHSR antagonist on LPS-induced pro-inflammatory cytokine productions in macrophages from C57/BL6 mice. C57/BL6 primary peritoneal macrophages were isolated and stimulated with LPS for 2 hours. Two doses of DLS (20 and 100 μM) were administrated to cells 24 hours before LPS stimulation. The productions of TNF (A), IL-6 (B) and IL-12 (p40) (C), were detected in the supernatants by ELISA. Each bar represents the mean ± SEM; NS: no significance, *p < 0.05 and ***p < 0.005.
