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. 2015 Mar 11;7(3):1798–1816. doi: 10.3390/nu7031798

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Procrustes rotation analysis comparing Unifrac phylogenetic distances from pyrotag sequencing and DGGE profiles of caecal communities of C57 and Il10^-/- mice in treatment groups SPF, C, EF, CIF and EF-CIF. Procrustes analysis is a method for rotating and scaling points from one ordination to be as close as possible to points from another ordination, while maintaining the relative distances between points within each ordination. In this instance the ordination points are Unifrac PCoA scores (tetrahedrons) and DGGE profile PCA scores. Lines separating points indicates similarity of Unifrac and DGGE analysis of the same sample, with short lines indicating greater similarity. Lines and points are coloured according to genotype and treatment group.