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. 2015 Mar 17;7(3):1978–1991. doi: 10.3390/nu7031978

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effect of ADL on serum leptin. Mice were fed normal-fat diet (NFD), high-fat diet (HFD), HFD supplemented with 100 mg·kg⁻¹·day⁻¹ ADL (HFD + ADL 100), HFD supplemented with 3000 mg·kg⁻¹·day⁻¹ ADL (HFD + ADL 3000), and HFD supplemented with 3000 mg·kg⁻¹·day⁻¹ yerba mate (HFD + MT 3000) for 6 weeks. Serum samples were obtained from 15 h-fasted mice. Leptin was assayed by ELISA. Bars represent mean ± SD (n = 3). Statistical significance was evaluated by Duncan post-hoc test in ANOVA. ^p < 0.001, compared to the high-fat diet group.

Inline graphic — Effect of ADL on serum leptin. Mice were fed normal-fat diet (NFD), high-fat diet (HFD), HFD supplemented with 100 mg·kg⁻¹·day⁻¹ ADL (HFD + ADL 100), HFD supplemented with 3000 mg·kg⁻¹·day⁻¹ ADL (HFD + ADL 3000), and HFD supplemented with 3000 mg·kg⁻¹·day⁻¹ yerba mate (HFD + MT 3000) for 6 weeks. Serum samples were obtained from 15 h-fasted mice. Leptin was assayed by ELISA. Bars represent mean ± SD (n = 3). Statistical significance was evaluated by Duncan post-hoc test in ANOVA. ^p < 0.001, compared to the high-fat diet group.