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. 2015 Mar 12;13(3):1267–1289. doi: 10.3390/md13031267

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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The cytotoxicity of different chitosan oligosaccharides in PC12 cells. PC12 cells were incubated with different chitosan oligosaccharides COS-2~4 (A), peracetylated chitosan oligosaccharides Q-2~4 (B) and N-acetylated chitosan oligosaccharides N-2~4 (C) at indicated concentrations (100, 200, 400 μg/mL) for 24 h. Then the cell viability was evaluated by resazurin assay. The results were presented as a percentage of non drug treated normal control group. Values are the mean ± SD (n = 3).