Table 2.
Mean ion intensity (n = 3) for secondary metabolites in the solvent wash area after separation from the hypobranchial gland tissue imprints on NALDI and DIOS nanostructured surfaces with corresponding LogP values (calculated using chemo-informatics software ChemBioDraw Ultra 13.0). Significant differences in the ion intensity for each compound were tested using univariate two factor PERMANOVA, followed by pairwise-tests.
| Compound | Log P | NALDI | DIOS | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Water | Ethanol | Chloroform | Hexane | Water | Ethanol | Chloroform | Hexane | ||
| Choline | −4.236 | 78,500 (±49,374) a | 25,067 (±16,050) b | N.D. | 19,500 (±7378) | 79,000 (±46,776) a | 19,500 (±7378) b | N.D. | 6264 (±2139) c |
| Murexine | −3.373 | 33,000 (±3812) a | 5320 (±1959) b | N.D. | N.D. | 33,067 (±3782) a | 1450 (±704) b | N.D. | N.D. |
| Tyrindoxyl sulfate ** | −0.346 | 2625 (±711) a | 22,133 (±2108) b | N.D. | N.D. | 1809 (±135) a,c | 5651 (±3922) c | N.D. | N.D. |
| 6-Bromoisatin * | 1.615 | N.D. | 360 (±213) a | 1775 (±725) b | N.D. | N.D. | 656 (±646) a | 3678 (±485) c | N.D. |
| Tyrindoleninone * | 2.889 | N.D. | 2506 (±1736) a | 2945 (±1277) a | N.D. | N.D. | 7957 (±2704) b | 2581 (±1311) a,b | N.D. |
| Tyrindolinone | 2.999 | N.D. | 424 (±107) | 958 (±233) | N.D. | N.D. | N.D. | 373 (±647) | N.D. |
| Tyrian purple (6,6′-Dibromoindigo | 4.47 | N.D. | N.D. | 6447 (±2093) | N.D. | N.D. | N.D. | 10848 (±4118) | N.D. |
| 6,6′-Dibromoindirubin) | |||||||||
| Cholesterol | 7.11 | 4722 (±755) | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
Different letters a, b & c indicate significant differences between each nanosurface and solvent combination (p < 0.05); Overall differences between the ion intensity on NALDI and DIOS are also indicated for each compound as p ≤ 0.05 (*) and p ≤ 0.01 (**); N.D. = not detected.