Figure 6.

Single-tube RT-PCR amplification of different splice variants. The investigated sample genes with alternative splicing were efg8, mgmt, lsg2, selEFf, nrnp1 and clpr2. Both splice variants of each gene were amplified in one and the same reaction using only a single pair of primers (Additional file 1: Table S3). The amplicons have been cloned and sequenced. The expected lengths of fragments are given in Additional file 1: Table S3. DNA fragments that are consistent with the predictions are marked by arrowheads.