Figure 2.

Quantification of platelet accumulation in renal allografts. (A) Digital planimetric measurements of P-selectin–stained platelets were about 28-fold higher after single transfer of DSA versus isotype control antibodies. After four repeated transfers, the area was about 5.6-fold higher in animals treated with alloantibody versus isotype control antibody. (B) Both PF4 and serotonin were greatly increased in allografts after single or multiple transfers of alloantibody compared with isotype control antibody. Each symbol represents data from an individual animal. AlloAb, alloantibody; IsoAb, isotype control antibody. *P<0.05 versus control; **P<0.01 versus control.