Table 3.
Proinflammatory cytokine and chemokine expression in TKPTS cells (control and after siRNA S1P1 knockdown) treated with vehicle, FTY720-p, cisplatin, or cisplatin+FTY720-p
| Control | siRNA Knockdown | |||||||
|---|---|---|---|---|---|---|---|---|
| Vehicle | FTY720-p | Cisplatin | Cisplatin+FTY720-p | Vehicle | FTY720-p | Cisplatin | Cisplatin+FTY720-p | |
| CXCL1 | 1.0±0.40 | 1.8±0.13 | 21.9±3.20a | 8.2±1.5b | 1.0±0.01 | 1.1±0.08 | 16.8±0.88a | 17.8±1.06 |
| MCP-1 | 1.0±0.02 | 0.9±0.05 | 3.19±0.2 | 1.9±0.08 | 1.0±0.03 | 1.2±-0.05 | 2.7±0.24 | 2.8±0.34 |
| IL-1β | 1.0±0.6 | 14.5±5.9 | 186.8±98.4a | 68.7±9.9b | 1.0±0.20 | 1.6±0.2 | 6.9±1.9 | 12.4±1.17 |
| IL-6 | 1.0±0.5 | 1.8±0.64 | 21.3±8.8a | 16.5±0.8 | 1.0±0.21 | 1.9±0.10 | 126.5±13.4a | 153.3±15.2 |
| TNF-α | 1.0±0.7 | 1.3±0.5 | 13.2±10.2a | 1.69±1.03 | 1.0±0.03 | 1.1±0.11 | 21.6±0.92a | 20.7±0.877 |
The left columns show data for untreated TKPTS cells, whereas the right columns show data for cells transfected with S1pr1 siRNA 48 hours before the experiment. Control (scrambled siRNA) or knockdown cells were incubated with vehicle (saline) or cisplatin (20 μm) for an additional 24 hours. mRNA expression relative to glyceraldehyde 3-phosphate dehydrogenase expressed as fold changes compared with respective vehicle. Values are the mean±SEM. n=3 in each group.
a
P<0.05 compared with respective vehicle.
b
P<0.05 compared with respective cisplatin.