Figure 2.

Knockdown of WNK1 expression decreases BKα protein expression in HEK BKα cells. HEK BKα cells were transfected with scramble control siRNA or a series of doses of siRNA WNK1. Forty-eight hours after transfection, cells were lysed and subjected to SDS-PAGE and Western blot analysis. (A) Representative immunoblots for BK, WNK1, and actin. (B) Bar graph shows mean±SEM band densities of BK from five independent experiments. Knockdown of WNK1 expression significantly decreases total BK protein expressions in a dose-dependent manner. n=5; *P<0.05 versus control.