Figure 3.

Overexpression of WNK1 increases BK protein expressions in HEK293 cells with transiently expressing BKα or stably expressing BKα. HEK293 cells were transfected with both pCMV-myc-BKα and a series of doses of pcDNA4.0-his-myc-WNK1 (A) in four independent experiments. HEK BKα cells were transfected with a series of doses of WNK1 in five independent experiments (B). Forty-eight hours after transfection, cells were lysed and subjected to SDS-PAGE and Western blot analysis. (A and B) Representative immunoblots for WNK1, BK, and actin. (C and D) Bar graphs of summarized data from respective immunoblots. Anti-myc antibody was used for detecting WNK1 and BK protein expressions in A. Anti-WNK1 and anti-BK antibodies were used to detect WNK1 and BK protein expressions, respectively, in B. Overexpression of WNK1 both in transiently transfected HEK293 cells and HEK stably expressing BKα cells significantly increased total BK protein expressions in a dose-dependent manner. *P<0.01 compared with the control.