Figure 2.

Selective depletion of monocytes and kidney macrophages in CD169-DTR mice. (A) White blood cells of DT-treated CD169-DTR mice at the indicated time points were stained with CD11b and Gr-1 antibodies. Numbers indicate the frequency (percentage) of CD11b⁺Gr-1^int-lo monocytes among white blood cells. Average percentages of CD11b⁺Gr-1^int-lo monocytes (three to five mice per group) are plotted with SD in B. Mean percentage of CD11b⁺Gr-1^int-lo cells was compared by one-way ANOVA. *P<0.05. (C) Kidney macrophages of DT-treated CD169-DTR mice at the indicated time points were stained for Gr-1, CD11b, and F4/80 antibodies. Numbers indicate the frequency (percentage) of CD11b⁺F4/80⁺ kidney macrophages among CD11b⁺ and/or CD11c⁺ cells in kidneys. Average percentages of CD11b⁺, F4/80⁺ kidney macrophages (four mice per group) are plotted with SD in D. Mean percentage of CD11b⁺F4/80⁺ cells was compared by one-way ANOVA. *P<0.05. (E) Depletion of monocytes in CX3CR1-GFP×CD169-DTR mice. DT was injected into CX3CR1-GFP mice (upper left panel) or CX3CR1-GFP×CD169-DTR mice (upper right panel). Twenty-four hours later, white blood cells were stained for CD11b. Numbers indicate the frequency (percentage) of CX3CR1^lo or CX3CR1^hi cells among white blood cells. (Lower panel) Average percentages of CX3CR1^lo and CX3CR1^hi cells are plotted. ▪, CX3CR1-GFP; □, CX3CR1-GFP × CD169-DTR. *P<0.05. (F) Depletion of kidney macrophages in CX3CR1-GFP × CD169-DTR mice. DT was injected into (left panel) CX3CR1-GFP or (right panel) CX3CR1-GFP×CD169-DTR mice. Two days after the DT injection, kidney macrophages were stained for CD11b and F4/80. Numbers indicate (upper panel) the frequency (percentage) of CX3CR1-positive cells among CD11b⁺ and/or CD11c⁺ cells in kidneys or (lower panel) the frequency (percentage) of CD11b and F4/80 double-negative, CD11b single-positive, or CD11b and F4/80 double-positive cells among CD11b⁺ and/or CD11c⁺ cells in kidneys. Data are representative of three independent experiments.