Figure 4.

AtGLR3.5 and calcium affect ABA sensitivity in seed germination. A, Calcium alleviates the inhibitory effect of ABA on germination. B, Phenotypes of 15-d-old wild-type (WT) and AtGLR3.5 RNAi seedlings treated with or without ABA (1 µm). C, Germination analysis of wild-type and AtGLR3.5 RNAi seeds treated with or without ABA (1 µm). D, Phenotypes of 9-d-old wild-type and AtGLR3.5 OE seedlings in the absence and presence of ABA (1 µm). E, Germination analysis of wild-type and AtGLR3.5 OE seeds in the presence and absence of ABA (1 µm). For the germination analysis in A, C, and E, seeds were scored for germination 36 h after incubation under the germination conditions, and data shown are means ± se of the mean (n = 3). In B and D, three independent experiments were performed and representative images are shown.