Biomass and roots-to-leaves ratio of mut4 and mut5 lines. A, Average biomass of leaves (gray bars) and roots (white bars) expressed as mg of fresh weight (FW) in 12-dpg plantlets grown in –P conditions (n = 30–80 plantlets). B, Average biomass of leaves (gray bars) and roots (white bars) expressed as mg of fresh weight in 12-dpg plantlets grown in +P conditions (n = 15–30 plantlets). C, Ratio of roots (R) to leaves (L; fresh weight biomass) in –P (white), +P (pale gray), and VHPi (10 mm ; dark gray) conditions. All experiments were reproduced at least twice. For A and B, bilateral Student’s t tests were conducted to assess statistical differences between the wild type (WT) and the other genotypes, comparing leaf biomass to wild-type leaf biomass and root biomass to wild-type root biomass. In A, the single asterisk indicates P < 0.001. In B, the single asterisk indicates P < 0.05.