Figure 7.

³³P uptake capacity in VHPi is mainly a passive process. A, Wild-type (WT) uptake capacity is reduced by CCCP addition, except in VHPi conditions. Wild-type plants were grown for 10 d in –P, +P, and VHPi before incubation in the corresponding media in control (white bars) or +10 μm CCCP (gray bars) conditions. B, In VHPi conditions, mut5 lines show the same uptake capacity as the wild type, both in the control (white bars) and with +CCCP addition (gray bars). Pi uptake capacity was calculated as nmol Pi h^–1 cm^–1 root. Data shown are from a representative experiment; experiments were performed in either duplicate or triplicate. Each bar corresponds to 20 to 24 plants. Error bars represent se.