Figure 8.

VHPi conditions restore transcriptional repression of PHT1 and PSI markers in mut5 lines. qRT-PCR of several members of the PHT1 family and several PSI genes in the wild type (WT), a mut4 line, and two independent mut5 lines. Plantlets were grown on +P as well as 2 and 10 mm Pi media (VHPi) for 11 d before RNA extraction from roots. Corresponding loci numbers and primer sequences are provided in Supplemental Table S2. Biological triplicates were performed, and all samples were analyzed with technical triplicates. sds are shown.