Table I. Low- and high-affinity kinetic constants ± sd of the wild type and double, quadruple, and quintuple mutant lines.
The Michaelis-Menten Km and Vmax were obtained by nonlinear regression analysis from data presented in Figure 4 using PRISM6 software.
| Genotype | High-Affinity Constants ± sd
|
Low-Affinity Constants ± sd
|
||
|---|---|---|---|---|
| Km | Vmax | Km | Vmax | |
| μm | nmol Pi h–1 cm–1 root | μm | nmol Pi h–1 cm–1 root | |
| Wild type | 6.67 ± 1,84 | 0.0920 ± 0.006 | 424 ± 209 | 0.327 ± 0.054 |
| pht1;1/pht1;4 | 6.04 ± 1.46 | 0.0409 ± 0.002 | 368 ± 263 | 0.114 ± 0.027 |
| mut4-1 | 9.07 ± 2.28 | 0.0121 ± 0.0008 | 362 ± 178 | 0.051 ± 0.008 |
| mut5-1 | 11.24 ± 3.17 | 0.0046 ± 0.0004 | 969 ± 892 | 0.029 ± 0.012 |