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. 2015 Feb 25;167(4):1307–1320. doi: 10.1104/pp.114.254078

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Figure 7. — Autophagy-dependent mobilization of Rubisco in the vacuole by RCBs in rice. A, Visualization of RCBs by GFP-labeled Rubisco in rice leaf. First leaves of OsRBCS2-sGFP-expressing rice were excised and observed immediately (0 h) or incubated for 20 h in the presence of 1 μm concanamycin A (+concA). B, Mobilization of GFP-labeled Rubisco is not observed in OsATG7 knockout plants. First leaves of OsATG7 knockout (Osatg7-1) or corresponding wild-type (OsATG7+/+) plants expressing OsRBCS-sGFP were excised and incubated for 20 h in the presence of 1 μm concanamycin A (+concA). GFP fluorescence appears green, and chlorophyll autofluorescence appears magenta. In merged images, overlapping signals appear white. In B, only merged images are shown. Bars = 10 μm.