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. 2015 Feb 3;167(4):1243–1258. doi: 10.1104/pp.114.254367

Figure 10.

Figure 10.

EMSAs show that AaNAC2 to AaNAC4 (N2–N4) bind to a 28-bp double-stranded DNA fragment of the AaTPS1 promoter (Aa) but not to the AcTPS1 promoter (Ac). A, Sequence alignment of the DNA probes used for the EMSA with the NAC core underlined. B, EMSAs of 3′-biotin-labeled double-stranded DNA probes with the AaNAC1-4 DNA binding domain proteins. Lane 1, Aa DNA probe only, no protein; lane 2, Ac DNA probe only, no protein; lanes 3 to 10, Aa or Ac DNA probe incubated with AaNAC1-4 protein combinations.