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. 2015 Feb 3;167(4):1243–1258. doi: 10.1104/pp.114.254367

Figure 5.

Figure 5.

Expression of MEP pathway genes during fruit ripening in A. arguta (black bars, ripening stages a0, a3, and a5; see text) and A. chinensis (gray bars, ripening stages c0, c2, and c4; see text). Real-time qPCR analyses were performed on pooled fruit samples using the gene-specific primers given in Supplemental Table S2. Error bars are based on four technical replicates, and data were normalized against the housekeeping gene EF1α. Error bars represent ±se. MEP pathway intermediate abbreviations are: CDP-ME, 4-diphosphocytidyl-methylerythritol; CDP-MEP, 4-diphosphocytidyl-methylerythritol 2-phosphate; DXP, deoxyxylulose 5-phosphate; GAP, glyceraldehyde 3-phosphate; HBMPP, hydroxymethylbutenyl 4-diphosphate; and ME-cPP, methylerythritol 2,4-cyclodiphosphate. Enzyme abbreviations in bold are: CMK, CDP-ME kinase (EC 2.7.1.148); CMS, CDP-ME synthase (EC 2.7.7.60); DXR, DXP reductoisomerase (EC 1.1.1.267); DXS, DXP synthase (EC 2.2.1.7); HDR, HMBPP reductase (EC 1.17.1.2); HDS, HMBPP synthase (EC 1.17.4.3); IDI, IDP isomerase (EC 5.3.3.2); MCS, ME-cPP synthase (EC 4.6.1.12); and ssGDPS, geranyl diphosphate synthase small subunit (EC 2.5.1.1). Enzymes considered to be rate determining according to the literature are boxed. *, TPS data are shown in Fig. 2, C and D.