Figure 2.

APX1 protein is S-nitrosylated. A, Detection of the S-nitrosylated His-APX1 recombinant protein (biotin-APX1; biotinylated proteins; for technical details, see “Materials and Methods”) in vitro. Treatments with GSH in the presence or absence of ascorbate sodium (Asc) are served as negative controls. B, Analysis of S-nitrosylation of APX1 in vivo. C, Accumulation of the S-nitrosylated APX1 in Col-0 and gsnor1-3 seedlings. A quantitative analysis of the data is shown on the right. Error bars = sd from three independent experiments (biological replicates). Two-tailed Student’s t test; **, P < 0.01.