Figure 1.

Dehydration and rehydration of C. pumilum. A typical dehydration/rehydration cycle of C. pumilum is shown. A hydrated plant (A) was dried out by withholding water. Anthocyanin pigment begins to accumulate in leaves at 40% to 50% RWC (B), and leaf surfaces are completely purple when RWC decreases further to approximately 15% (C). At 4 h (D) and 24 h (E) after watering, the plant was rehydrated and returned to its state prior to drying. Images of leaf cross sections from a hydrated plant (F) and a plant at 10% to 15% RWC (G) also are shown. Note the presence of anthocyanin in both the top and bottom epidermis of the dry leaf (G). Bars = 500 µm.