Figure 7.
Protein topology of AXY9. A, Split YFP protein fusions, AXY9-YFPN and AXY9-YFPC, were coexpressed with the complementary half of the YFP expressed inside (TMD-YFPN/C) or outside (YFPN/C-TMD) of the Golgi in N. benthamiana and examined using confocal microscopy. B, Protease protection assay using microsomes prepared from N. benthamiana transiently expressing the AXY9-GFP fusion protein. A western blot using an anti-GFP antibody was used to detect the fusion peptide after digestion with (+) or without (−) protease and detergent.