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. 2015 Feb 19;167(4):1643–1658. doi: 10.1104/pp.15.00049

Figure 2.

Figure 2.

Subcellular localization and dimerization of GRXS17. A, Transient expression of a GRXS17:GFP fusion in Arabidopsis mesophyll protoplasts. Autofluorescence of chlorophyll indicates chloroplasts. B, Stable expression of a GRXS17:GFP fusion in Arabidopsis leaves. White arrows indicate nuclei. C, Immunodetection of GRXS17, TRXh5, and nucleolin NUC1 in cytosolic (Cyt) and nuclear (Nuc) extracts of Arabidopsis flower buds. D, BiFC assay of GRXS17. Vectors encoding C- and N-terminal split-YFP fusions with GRXS17 were cotransformed into Arabidopsis mesophyll protoplasts. E, Immunodetection of GRXS17 in grxS17 and wild-type extracts cross-linked (C) or not (NC) with DMP. WT, Wild type.