Rescue of the Baker’s yeast grx5 mutant defects by AtGRXS17. A, Sensitivity to tert-butyl hydroperoxide (t-BOOH) or diamide. The indicated Baker’s yeast strains were spotted on YPD plates at 10× serial dilutions and grown for 3 d at 30°C on YPD plates. B, Growth on Glc (YPD plates) or glycerol (YPGly plates) after 3 d at 30°C. C, Relative ratio between aconitase and malate dehydrogenase (MDH) activities normalized with respect to the ratio in the wild-type strain in exponential cultures at 30°C in yeast extract-peptone-Gal medium. D, Relative specific Leu-1 activity. E, Northern-blot analysis of FIT3 and FTR1 mRNA levels from exponential cultures at 30°C. Loading control: SNR19 (for Small Nuclear RNA). F, Relative iron content from exponential cultures at 30°C in YPD medium. Mean ± sd (n = 3). ACO, Aconitase; WT, wild type. *, Statistically significant differences (Student’s t test, P < 0.05) compared with wild-type (GRX5) cultures.