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Immunoblot analysis of the wild type (cw15-325), NAC2, and ΔPSAB. The wild type and NAC2 were grown in Tris-acetate-phosphate (TAP) medium in low light (LL; approximately 30 μE m−2 s−1). ΔPSAB was grown in TAP in very LL (approximately 6 μE m−2 s−1) conditions. Two micrograms of chlorophyll of the whole-cell extracts was loaded on a 13% SDS-PAGE gel and analyzed by immunoblotting using anti-PSBA, anti-PSAD, and anti-PSBR antibodies.
