Figure 2.

Comparative quantitative reverse transcription (RT)-PCR and immunoblot analysis of psbr RNA and protein complement in the wild type (WT) and PSBR knockdown mutants. A, PSBR expression is significantly down-regulated in PSBR knockdown mutants as revealed by RT-PCR. β-Tubulin was used as a loading control. PSBR expression level is expressed as mean PSBR/β-tubulin ± sd; n = 3. B, Thylakoids isolated from high light (HL)-treated (24 h) cells were separated on a 13% (w/v) SDS-PAGE gel and analyzed by immunoblotting using anti-PSBR antibodies. Six micrograms of chlorophyll equals 100%, and ATPB served as a loading control.