Figure 5.

SDGs of thylakoid membranes from the wild-type and PSBR knockdown strains. A, Cultures (the wild type [WT], ami-RNA-psbr-1, and ami-RNA-psbr-2) were initially grown in TAP medium under LL (20 μE m⁻² s⁻¹) and then treated with HL (200 μE m⁻² s⁻¹) in HSM for 2 and 24 h. Isolated thylakoid membranes (200 μg of chlorophyll) were solubilized with 1% (w/v) α-DM and separated by SDG ultracentrifugation. B, SDGs of 2 h of HL were fractionated from the bottom to the top (fractions 1–30), and absorption at 675 nm was measured. SDG fractions are labeled according to their protein complex composition. The green SDG fraction obtained after 2 h of HL between LHCII and PSI-LHCI is probably composed of PSII core particles.