Down-regulation of PSBR affects PSII-LHCII-LHCSR3 supercomplex formation. A, Polypeptides from the 2-h HL
SDG fractions 3 to 16 of the wild type (WT) and ami-RNA-psbr-2 were analyzed by immunoblotting with antibodies against LHCSR3, PSAD, PSBA, and PSBR. Sixty microliters of each fraction was loaded per lane. B, Two-hour fractions (8–10 and 18–26) of the wild type and ami-RNA-psbr-2 were subjected to immunoblotting with an anti-LHCSR3, anti-PSBA, or anti-PSBR antibody. Sample preparation was performed as in A.