Figure 9.

Immunoblot analysis of whole cells and SDG fractions 2 to 28 from 24 h of HL and comparative quantitative proteomic analysis of polypeptides of the PSII supercomplex and ATPase from the wild type (WT) and FUD39. A, Cell cultures of the wild type and FUD39 were grown in TAP in HL for 24 h. Whole-cell extracts were loaded on a 13% (w/v) SDS-PAGE gel and analyzed by immunoblotting using anti-PSBA, anti-LHCSR3, anti-PSAD, and anti-PSBR antibodies. Three micrograms of chlorophyll equals 100%, and ATPB served as a loading control. B, SDG fractions 2 to 28 from 24 h of HL (the wild type and FUD39) were subjected to immunoblotting with anti-PSBA, anti-LHCSR3, anti-PSAD, and anti-PSBR antibodies. Sixty microliters of each fraction was loaded per lane. C, The same volume (100 μL) of PSII supercomplex fractions from 24 h of HL SDGs (the wild type and FUD39) was analyzed by mass spectrometry. The ratios of ¹⁴N-labeled wild-type and ¹⁵N-labeled FUD39 polypeptides are presented (mean values ± sd).