Figure 4.

TuMV replication complexes in xylem vessel. Longitudinal sections of N. benthamiana stem internodes infected with TuMV expressing 6K₂ fused to either GFP (A and C–E) or mCherry (B) were observed with a Zeiss LSM-780 confocal microscope with a 63× objective. Lipids were stained with the membrane dye DiOC₆(3) (B). Immunohistolocalization of dsRNA (C), vRdRp (D), and CP (E) was performed as described in Figure 3. The Fluorescent Brightener 28-stained cell wall is shown in false-color magenta, 6K₂:GFP is shown in green, 6K₂:mCherry has been false colored in green, and DiOC₆(3) has been false colored in red. dsRNA, vRdRp, and CP are shown in red. A is a three-dimensional image; the other confocal images are single optical slices.