Figure 9.

Membrane-associated vRNA in PVX-infected sieve elements and xylem vessels. Longitudinal sections of PVX-infected N. benthamiana stem internode above the inoculated leaf were collected by cryosectioning and observed by confocal microscopy. Immunohistolocalization of dsRNA and lipid staining with DiOC₆(3) in one sieve element (A) and one xylem vessel (B) of a PVX-infected plant are shown. Aniline Blue-stained callose and Fluorescent Brightener 28-stained cell wall are shown in false-color magenta, DiOC₆(3) is shown in green, and dsRNA is shown in red. All images are single optical slices.