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. 2015 Mar 15;29(6):585–590. doi: 10.1101/gad.256354.114

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© 2015 Alabert et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Recycling of histone variants at replication forks. Experimental design as in Figure 1A except that nascent chromatin was collected in early (E), mid- (M), and late (L) S phase (Supplemental Fig. S3A). (A) Unique peptides used to differentiate canonical histones H3.1 and H3.2 from the H3.3 variant as well as histone H2A from H2A.X and H2A.Z variants. (B) Enrichment of H3.3 relative to total H3 (H3.3+H3.1/2). (C,D) Enrichment of H2A.X and H2A.Z relative to total H2A (H2A+H2A.Z+H2AX). (E) Enrichment of H2A.Z in labeled chromatin at 0 h (nascent; mid-S phase) and 10 h later (see Supplemental Fig. S4A for experimental design). Error bars indicate SD. 3 < n < 9. Unpaired t-test: (***) P < 0.001; (**) P < 0.05; (n.s.) nonsignificant.