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. 2015 Mar 15;29(6):591–602. doi: 10.1101/gad.257626.114

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© 2015 Kapoor et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — SWI/SNF specifically functions in S phase to promote Mec1 activity. (A) Western blot analysis of Rad53 phosphorylation in G1 cells. Wild-type (WT) and Δsnf2 cells were arrested in G1 phase with α factor and treated with 4NQO. (B) Western analysis of Rad53 phosphorylation in G2 cells. Wild-type and Δsnf2 cells were arrested in G2 phase with nocodazole and treated with 4NQO. (C) Western analysis of Rad53 phosphorylation in S phase. Wild-type and Δsnf2 cells were arrested in G1 and then released into medium containing 200 mM HU.