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. 2014 Dec 16;15(1):1122. doi: 10.1186/1471-2164-15-1122

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© Rong et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression patterns of differentially expressed miRNAs and one target gene (tetur0906680) in the female and male comparisons. A. and B. Nine common differentially expressed miRNAs in the comparisons FI vs FU (control) and MI vs MU (control) identified by using Illumina small RNA deep sequencing (log2 fold-change) and validated by qRT-PCR. C. Validation of five female-specific differentially expressed miRNAs by qRT-PCR. B and C. Error bars represent standard errors of averages from three biological replicates (NS: not significant; *P < 0.05; **P < 0.01; ***P < 0.001; Fisher's LSD test). D. qRT-PCR and Illumina small RNA deep sequencing both showed that candidate target gene tetur09g06680 of novel_16 was up-regulated in Wolbachia-infected mites. All the experiments had three biological replicates.