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. 2014 Aug 5;115(6):2240–2254. doi: 10.1021/cr5002904

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Copyright © 2014 American Chemical Society

This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.

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Detection of 5mC and 5hmC with restriction endonucleases. 5mC and 5hmC can be distinguished at an HpaII/MspI restriction digestion site by comparing digestion reactions. HpaII only digests DNA that does not contain 5mC or 5hmC. MspI digests DNA that contains 5mC and 5hmC. Glucosylation of 5hmC (5gC) inhibits digestion with MspI. 5hmC can be distinguished by comparing glucosylation/MspI digestion and MspI only digestion. 5mC can be distinguished by comparing glucosylatin/MspI digestion and HpaII digestion.