Skip to main content
. 2015 Apr 1;142(7):1375–1386. doi: 10.1242/dev.117093

Fig. 3.

Fig. 3.

Efficient generation of striatal projection neurons by activin stimulation. (A) Experimental scheme. (B) qPCR analysis for striatal projection neuron-expressed genes and neuronal transmitter genes. *P≤0.05, **P≤0.01, Student's t-test. (C-I) Immunostaining of day 36-40 cultures for the indicated markers. (G) DARPP32 with a high-magnification inset showing dendrites. (J) Quantification of immunostaining in D,E, showing the percentage of DARPP32+ neurons. (K) Quantification of other neuronal subtypes. ***P≤0.001, Student's t-test between control and activin-treated groups for hPSC lines in J and TBR1 and CR in K. (L) Minimal requirement for activin as evaluated by DARPP32+ neuron production. **P≤0.01, two-way ANOVA. Scale bars: 25 µm in G; 40 µm in C left, F,H; 75 µm in C right, D,E,I.