Fig. 3.

FMRP is required for activity-dependent MVP2 dendritic development. Dorsoventral series of MVP2 dendritic arbors with Oli-Gal4-driven UAS-ChR2(H134R) depolarization during day 1 post eclosion. Representative images show vehicle-fed wild-type control (A), ATR-fed wild-type experimental (B), vehicle-fed dfmr1^50M null (C) and ATR-fed dfmr1^50M null experimental (D) conditions following 24 h of 5 Hz blue light stimuli. On the right, dendritic arbors are shown in a dorsoventral series of 3 µm steps through the z-stack images. Scale bars: 10 μm. (E) Quantification of dendritic arbor volume in all four conditions. N values are as follows: wt^ETOH, 25; wt^ATR, 31; null^ETOH, 32; null^ATR, 25. ***P<0.001; n.s., not significant (ANOVA).