Fig. 3.

Inhibition of chondrogenesis in mutants at E14.5. (A-H) Alcian Blue and Nuclear Fast Red staining of E14.5 controls (A,B,E,F) and mutants (C,D,G,H). The level of Alcian Blue-positive mucin was reduced in mutant nasal cartilage (D,H, arrows). (I-P) H&E staining of E14.5 controls (I,J,M,N) and mutants (K,L,O,P). Mutants had a shorter nasal septum (M,O, brackets) and cartilage defects in both anterior and posterior nasal cavity levels (L,P, arrowheads). Boxed areas in A,C,E,G,I,K,M, and O are shown at higher magnification in B,D,F,H,J,L,N and P, respectively. Scale bars: 200 µm in A,B,F,I,J,N; 1 mm in E,M.