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. 2015 Apr 1;142(7):1368–1374. doi: 10.1242/dev.118968

Fig. 4.

Fig. 4.

MS2-tagged nos3′utr colocalizes with endogenous nanos3 and a subset of Vasa-positive germ granules in PGCs. (A,B) Endogenous nanos3 RNA localizes to PGCs at 30 hpf and is present throughout the cytoplasm and in larger perinuclear accumulations (arrows). Scale bar: 10 µm. (C-F) Cherry-positive cells at 30 hpf have diffuse cytoplasmic MCP-GFP signal and larger perinuclear accumulations (arrows). Scale bar: 10 µm. (G-J) Accumulations of MCP-GFP in Cherry-expressing PGCs at 30 hpf in cherry-24xMBS-nos3′UTR-injected embryos (white arrows) colocalize with endogenous nanos3 accumulations. Nonspecific nuclear aggregates of MCP-GFP (yellow arrowheads) do not colocalize with endogenous nanos3, consistent with these structures being artifacts. Scale bar: 25 µm. (K-N) A subset of MCP-GFP accumulations in Cherry-expressing PGCs at 30 hpf in cherry-24xMBS-nos3′UTR-injected embryos coincide with Vasa protein-positive germ granules (arrows) whereas others do not (arrowheads). Scale bar: 10 µm. The dotted lines denote borders of cells expressing Cherry reporter.