Fig. 8.

Depletion of Gas1 modulates FGF signaling through AKT in vitro and in vivo. (A) Western blots of whole-cell lysates from LB-22 cells treated with Gas1 siRNA or scramble control and stimulated with 50 ng/ml FGF as indicated. (B) Densitometry of repeat western blots as in A. Results are normalized to the non-stimulated, vehicle-treated control and to total AKT, total ERK or tubulin, respectively. Data show the mean±s.e.m., n≥3; ns, not significant. (C-G) Immunohistochemistry for phospho-Akt at E15.5 shows diminished phospho-Akt signal in condensed mesenchyme upon Gas1 knockout. Black boxes in C,D correspond to high-power panels. Ureteric tips are labeled with asterisks. Arrowheads indicate condensed mesenchyme. Scale bars: 200 µm (low power), 25 µm (high power), 75 µm (mid power).