Skip to main content
. 2014 Jul 29;20(4):459–471. doi: 10.1038/mp.2014.69

Figure 4.

Figure 4

Apc4 plays a main role in the proliferation and horizontal expansion of neural stem progenitor cells (NSPCs). (a) Proliferation of NSPCs from E14 nestin-Cre conditional Pqbp1-knockout (cKO) embryos was retarded in primary culture but rescued by transfection of Apc4 (n=4). **P<0.01 in one-way analysis of variance (ANOVA) with post hoc Tukey's test. (b) Fluorescence-activated cell sorting analysis showed G2/M accumulation and a mild increase in the G1 population in NSPCs derived from E14 cKO embryos. Apc4 transfection rescued delayed proliferation and prevented cyclin B accumulation in NSPCs from cKO embryos. (c) The pial-to-apical surface area ratio reflects the proliferation of in-utero transfected EGFP-positive cells after a defined time period. pApc4-IRES-hrGFPII or pIRES-hrGFPII were electroporated into the ventricular zone of E13 embryos (nestin-Cre control and cKO mice), and the brains were analyzed at E18. (d) Pial-to-apical ratio was deduced from 3D reconstruction of the rostral-to-caudal axis serial sections of six embryos in each genotype. The ratio was decreased in Pqbp1-cKO embryos, reflecting the decreased cell cycle times of NSPCs, but it was rescued by Apc4 expression. **P<0.01 or *P<0.05 in one-way ANOVA with post hoc Tukey's test.