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Osteogenic differentiation of MG63 cells.
Notes: (A) ALP, COL, and mineralization by BCIP/NBT, Sirius Red and Alizarin Red staining respectively (scale bar =200 μm). (B–D) Quantitative information of (A) by Image J analysis. *P<0.05 compared to naked Ti.
Abbreviations: ALP, alkaline phosphatase; COL, collagen; CS, chitosan; LbL, layer-by-layer; siCkip-1, osteogenic siRNA targeting Ckip-1; siNC, siRNA negative control.
