Figure 1.

Experimental timeline. (A) To determine the effect of treatment duration on cognitive recovery, injured mice received BCAA supplemented drinking water or untreated water for varying lengths of time. Vertical arrows indicate times for injury, treatment onset, and conditioned fear testing. (B) To determine the duration of action of BCAA treatment, mice receiving 10 consecutive days of BCAA therapy were compared to mice receiving 5 days of BCAA supplemented water followed by 5 days of untreated water. (C) To determine the effective concentration of BCAA supplemented water, mice consumed 5 days of either 100 or 50 mM BCAA supplemented water or untreated water. Abbreviations: FPI, fluid percussion injury; CFR, conditioned fear response, BCAAs, branched chain amino acids.