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. 2014 Dec 15;15(1):1104. doi: 10.1186/1471-2164-15-1104

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© Chen et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Alterations in RE accessibility during ESC differentiation. (A) Brightfield images of control (EGFP) or Oct4 KD ESC colonies indicate colony flattening and elongated cellular morphology upon Oct4 depletion. (B) Western blot of Oct4 in control (EGFP) or Oct4 KDs, indicating KD efficiency. RNA Polymerase II blot (Pol II) is shown as a loading control. (C, E) Average RE accessibility upon EGFP or Oct4 KD is shown relative to DHSs (C), or CTCF binding sites (E). (D, F) MNase-seq data. Nucleosome occupancy over DHSs (D), or CTCF binding sites (F). RED-seq and MNase-seq data are plotted as in Figure 3.