Figure 7.

Proinflammatory cytokines production. Peritoneal macrophages were obtained and exposed in vitro to 2.9, 29, and 290 μg/mL of Bxb as described above. At different times, the levels of IL-6 and IFN-γ were determined by ELISA assay using monoclonal antibodies as the probe. The levels of TNF were determined by standard assay with L929 cells. Each point represents the mean value of samples from four experiments in different groups of five mice. Statistical differences between the treatments were P < 0.05.