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Immunostaining and RT-PCR of pluripotency genes for biPS-1. (A) Immunostaining of OCT4, SSEA-1, SSEA-3, and SSEA-4 (brightfield (BF); blue, Hoechst33342 nuclear counterstain; red and yellow, antibody staining); controls, without first antibody, Bars, 100 μm. (B) RT-PCR analysis of biPS-1 cells. M, marker; BFF, bovine fetal fibroblasts; biPS-1, bovine iPS line; -RT, no RT.
