. 2015 Apr 1;17(2):131–140. doi: 10.1089/cell.2014.0080

Table 2.

Sleeping Beauty and piggyBac Transposon Reprogramming of Bovine Fibroblasts

	bFGF (4 ng/mL)				bFGF (4 ng/mL)+2i				hLIF (1000 U/mL)+2i				bFGF (8 ng/mL)+hLIF (1000 U/mL)				bFGF+hLIF
Transposase	PB			SB	PB			SB	PB			SB	PB			SB	—–	—–	—–
Transposon type	3F	4F	6F	4F	3F	4F	6F	4F	3F	4F	6F	4F	3F	4F	6F	4F	3F	4F	6F
No. of replicates	8	8	8	8	8	8	8	8	8	8	8	8	8	8	8	8	4	4	4
Colony formation rate (%)	0.006	0.005	0.014	0.003	0.003	0.010	0.021	0.012	0.003	0.018	0.006	0.009	0.005	0.012	0.024	0.011	0	0	0
No. of colonies picked	14	16	19	12	15	19	20	16	11	16	10	18	18	14	21	17	0	0	0
No. of opaque/nonproliferating colonies found	42	37	28	54	51	48	33	36	28	47	39	34	56	52	46	57	54	26	40
Stable iPSC line/maximal passage	0	0	1/P12^a	0	0	0	1/P18^a	0	0	0	0	0	0	0	1/P40^b	0	0	0	0
AP expression	—	—	No	—	—	—	Yes	—	—	—	Yes	—	—	—	Yes	—	—	—	—
Pluripotency gene expression (RT-PCR)	—	—	No	—	—	—	No	—	—	—	No	—	—	—	Yes	—	—	—	—
Teratoma formation	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	4/6	NA	NA	NA	NA

^{^a}

Cells ceased proliferation.

^{^b}

Culture with ongoing proliferation.

bFGF, basic fibroblast growth factor; hLIF, human leukemia inhibitory factor; PB, piggyBac transposon system; SB, Sleeping Beauty transposon system; iPSC, induced pluripotent stem cell; AP, alkaline phosphatase; NA, not assessed.