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. 2015 Mar 9;112(12):3728–3733. doi: 10.1073/pnas.1422455112

Fig. 1.

Fig. 1.

FC loading increases SPT activity. (A) Cellular FC levels and (B) cellular SM levels, in RAW264.7 cells unloaded or loaded with 100 µg/mL AcLDL, 100 µg/mL AcLDL + 2 µg/mL ACATi, or 50 µg/mL cholesterol-cyclodextrin (Chol-CD) for 16 h (n = 3, mean ± SD, ***P < 0.005 vs. control, by ANOVA posttest). (C) SPT activity of RAW264.7 cells unloaded or loaded with 100 µg/mL AcLDL, in the absence or presence of 2 µg/mL ACATi for 16 h at 37 °C. Myriocin was used as negative control (n = 3, mean ± SD, different numbers above the bars show P < 0.05, by ANOVA posttest). (D) SPT activity of RAW264.7 cells after a 16-h incubation with or without 50 µg/mL cholesterol-CD (n = 3, mean ± SD, ***P < 0.005, by t test).