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. 2015 Mar 9;112(12):3841–3846. doi: 10.1073/pnas.1502971112

Fig. 2.

Fig. 2.

Chromatin regulators enriched in the histone mark ChIP-MS preparations in mESCs. (A) Number of known, implicated, and novel chromatin-associated proteins that were enriched in the histone mark preparations. (B) Known (blue), implicated (gray), and novel (orange) chromatin-associated proteins. (C) Relative enrichment of selected chromatin regulators in various ChIP preparations. (D) Relative enrichments of PHIP, UBR7, and USP48 in various ChIP preparations. Histone mark ChIP followed by Western blot analysis of PHIP, UBR7, and USP48; 0.25% of input was loaded for ChIP Western blot. (E) ChIP-qPCR analysis of PHIP, UBR7, and USP48 with primers targeting enhancers (E), promoters (P), and gene body regions (G1 and G2) of Zfml and Agfg1. NC, negative control region for qPCR. Error bars indicate SD based on two independent experiments.