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Synthesis of mono-ADP-ribosylated peptides. (a) On-resin phosphorylation and AMP conjugation of an orthogonally protected ribosyl moiety. (b) Chemoselective ADP-ribose (inset) ligation to aminoxy-functionalized peptides. (c) ADP-ribose conjugates of N-methyl aminoxy-functionalized peptides retain the ribo-furanosyl-form. AMP = adenosine monophosphate, ADP = adenosine diphosphate.
